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goat anti gal 3  (R&D Systems)


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    Structured Review

    R&D Systems goat anti gal 3
    Goat Anti Gal 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+gal+3/Human+Galectin-3+Antibody/pm38377735-68-27-31
    Average 93 stars, based on 25 article reviews
    goat anti gal 3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Apolipoprotein E4 impairs the response of neurodegenerative retinal microglia and prevents neuronal loss in glaucoma.
    Article Snippet: In brief The apolipoprotein E4 (APOE4) allele is associated with an increased risk of Alzheimer disease and a decreased risk of glaucoma, but the underlying mechanisms remain poorly understood.. Margeta et al. demonstrate that APOEGalectin-3 signaling regulates cytotoxic neurodegenerative retinal microglia in glaucoma and that the APOE4 allele attenuates microglial neurodegenerative phenotype and protects neurons in glaucoma.

    Western Blot:

    Article Title: Downregulating Galectin-3 Inhibits Proinflammatory Cytokine Production by Human Monocyte-Derived Dendritic Cells
    Article Snippet: Recombinant cytokines: recombinant human GM-CSF, IL-1β, IFN-γ, TNF-α (BioLegend, San Diego; PreproTech, Rocky Hill, NJ); recombinant human IL-6, human SCF, human TSLP (PreproTech); PGE2 (Sigma, St Louis, MO); recombinant human Gal-3; ELISA kits for human cytokines : IL-1β, TGF-β, p19 (IL-23), p35 (IL-12), IL-10, IL-17A, and were obtained from eBioscience and PreproTech; anti-actin (Sigma). .. Cytokine neutralizing antibodies (used from 5–10 μg/ml): anti-IL-12 (eBioscience); anti-human IL-12 p35, anti-IL-12/IL23 p40, anti-IL-23 p19, anti-IL-12 p70, anti-human IFN-γ, IL-β, IL-6, TGF-β1 (R&D); goat anti-Gal-3 (pAb, R&D), biotin mAb anti-Gal-3 (M3/38, BioLegend for western), mAb anti-Gal-3 (B2C10, prepared by this lab; and inhibition of radioactive Gal-3 binding to solid phase IgE by β-galactosides was previously described in the lab [ ]. .. FACS reagents: FITC-anti-p35, PE-anti-p19, APC-anti-CD83, PE anti-CD205 (DEC-205) (BioLegend); PE-anti-CD8α, FITC anti-human CD11c, APC anti-human CD11c, Brefeldin A, eFluor 710 streptavidin, and appropriate fluorochrome-matched control antibodies (eBioscience); FITC anti-human Gal-3 (BioLegend), APC anti-human Gal-3 (R&D Systems).

    Inhibition:

    Article Title: Downregulating Galectin-3 Inhibits Proinflammatory Cytokine Production by Human Monocyte-Derived Dendritic Cells
    Article Snippet: Recombinant cytokines: recombinant human GM-CSF, IL-1β, IFN-γ, TNF-α (BioLegend, San Diego; PreproTech, Rocky Hill, NJ); recombinant human IL-6, human SCF, human TSLP (PreproTech); PGE2 (Sigma, St Louis, MO); recombinant human Gal-3; ELISA kits for human cytokines : IL-1β, TGF-β, p19 (IL-23), p35 (IL-12), IL-10, IL-17A, and were obtained from eBioscience and PreproTech; anti-actin (Sigma). .. Cytokine neutralizing antibodies (used from 5–10 μg/ml): anti-IL-12 (eBioscience); anti-human IL-12 p35, anti-IL-12/IL23 p40, anti-IL-23 p19, anti-IL-12 p70, anti-human IFN-γ, IL-β, IL-6, TGF-β1 (R&D); goat anti-Gal-3 (pAb, R&D), biotin mAb anti-Gal-3 (M3/38, BioLegend for western), mAb anti-Gal-3 (B2C10, prepared by this lab; and inhibition of radioactive Gal-3 binding to solid phase IgE by β-galactosides was previously described in the lab [ ]. .. FACS reagents: FITC-anti-p35, PE-anti-p19, APC-anti-CD83, PE anti-CD205 (DEC-205) (BioLegend); PE-anti-CD8α, FITC anti-human CD11c, APC anti-human CD11c, Brefeldin A, eFluor 710 streptavidin, and appropriate fluorochrome-matched control antibodies (eBioscience); FITC anti-human Gal-3 (BioLegend), APC anti-human Gal-3 (R&D Systems).

    Binding Assay:

    Article Title: Downregulating Galectin-3 Inhibits Proinflammatory Cytokine Production by Human Monocyte-Derived Dendritic Cells
    Article Snippet: Recombinant cytokines: recombinant human GM-CSF, IL-1β, IFN-γ, TNF-α (BioLegend, San Diego; PreproTech, Rocky Hill, NJ); recombinant human IL-6, human SCF, human TSLP (PreproTech); PGE2 (Sigma, St Louis, MO); recombinant human Gal-3; ELISA kits for human cytokines : IL-1β, TGF-β, p19 (IL-23), p35 (IL-12), IL-10, IL-17A, and were obtained from eBioscience and PreproTech; anti-actin (Sigma). .. Cytokine neutralizing antibodies (used from 5–10 μg/ml): anti-IL-12 (eBioscience); anti-human IL-12 p35, anti-IL-12/IL23 p40, anti-IL-23 p19, anti-IL-12 p70, anti-human IFN-γ, IL-β, IL-6, TGF-β1 (R&D); goat anti-Gal-3 (pAb, R&D), biotin mAb anti-Gal-3 (M3/38, BioLegend for western), mAb anti-Gal-3 (B2C10, prepared by this lab; and inhibition of radioactive Gal-3 binding to solid phase IgE by β-galactosides was previously described in the lab [ ]. .. FACS reagents: FITC-anti-p35, PE-anti-p19, APC-anti-CD83, PE anti-CD205 (DEC-205) (BioLegend); PE-anti-CD8α, FITC anti-human CD11c, APC anti-human CD11c, Brefeldin A, eFluor 710 streptavidin, and appropriate fluorochrome-matched control antibodies (eBioscience); FITC anti-human Gal-3 (BioLegend), APC anti-human Gal-3 (R&D Systems).

    Incubation:

    Article Title: Interplay in galectin expression predicts patient outcomes in a spatially restricted manner in PDAC.
    Article Snippet: FFPE pancreas tissue Section 5 mm in depth were subjected to deparaffinisation using histoclear (Sigma) followed by incubation in decreasing concentrations of ethanol prior to antigen retrieval using a 10 mM citrate buffer at pH6 for 15 min. .. Slides were blocked using 10% normal horse serum in 2% BSA/PBS for 10 min, prior to incubation at 4◦C overnight with goat anti-Gal-1 (1:75, AF1152, R&D systems), goat anti-Gal-3 (1:75, AF1154, R&D systems) or goat anti-Gal-4 (1:20, AF1227, R&D systems) antibodies diluted in PBA with 1% BSA. .. Following 3 ×5-min washes in PBS, slides were incubated for 45 min in the dark at room temperature with donkey-anti-goat IgG AF647 (1:400, A-21447, Invitrogen).



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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    R&D Systems goat anti mouse gal 3 antibody
    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    Image Search Results


    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.

    Journal: Upsala Journal of Medical Sciences

    Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

    doi: 10.48101/ujms.v126.7673

    Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.

    Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

    Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay

    Surface-associated glycoproteins and gamma-glutamyl transferase on seminal prostasomes of oligozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of oligozoospermic men (sPro-O) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-O from Sephadex G-200 column (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. Barely detectable CD9- and gal-3-immunoreactivity is indicated by circle. A450, absorbance at 450 nm; GGT, gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3, galectin 3; F, fraction.

    Journal: Upsala Journal of Medical Sciences

    Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

    doi: 10.48101/ujms.v126.7673

    Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on seminal prostasomes of oligozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of oligozoospermic men (sPro-O) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-O from Sephadex G-200 column (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. Barely detectable CD9- and gal-3-immunoreactivity is indicated by circle. A450, absorbance at 450 nm; GGT, gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3, galectin 3; F, fraction.

    Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

    Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay