goat anti gal 3  (R&D Systems)

Journal: Upsala Journal of Medical Sciences

Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

doi: 10.48101/ujms.v126.7673

Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.

Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay

Journal: Upsala Journal of Medical Sciences

Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

doi: 10.48101/ujms.v126.7673

Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on seminal prostasomes of oligozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of oligozoospermic men (sPro-O) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-O from Sephadex G-200 column (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. Barely detectable CD9- and gal-3-immunoreactivity is indicated by circle. A450, absorbance at 450 nm; GGT, gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3, galectin 3; F, fraction.

Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay